Expanding virus susceptibility spectrum of MDBK cells by expressing host receptors nectin 4 and TfR.

ABSTRACT: Cell-based vaccine manufacturing is a flexible and cost-effective approach for vaccine production which, however, requires cell adaptation to new vaccine strains. Generating one omnipotent or semi-omnipotent cell line feasible for the production of multiple viruses could help resolve this problem. We previously proposed virus Baltimore subtyping-based choice of receptors and a panel of minimally preferred receptors for the establishment of cells with a broad virus susceptibility spectrum. With the aim of establishing cells sensitive to viruses of livestocks including bovine, ovine and canine, we selected TfR and Nectin 4 from the minimally preferred receptor panel, and successfully sensitized the starting cell line MDBK to CPV and CDV infection. Our study is a preliminary validation of our

SUBMITTER: Han P 

PROVIDER: S-EPMC7832251 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature