A comparison of SARS-CoV-2 wastewater concentration methods for environmental surveillance.

ABSTRACT: Wastewater¹ surveillance of SARS-CoV-2 may be a useful supplement to clinical surveillance as it is shed in feces, there are many asymptomatic cases, and diagnostic testing can have capacity limitations and extended time to results. Although numerous studies have utilized wastewater surveillance for SARS-CoV-2, the methods used were developed and/or standardized for other pathogens. This study evaluates multiple methods for concentration and recovery of SARS-CoV-2 and seeded human coronavirus OC43 from municipal primary wastewater and/or sludge from the Greater Seattle Area (March-July 2020). Methods evaluated include the bag-mediated filtration system (BMFS), with and without Vertrel™ extraction, skimmed milk flocculation, with and without Vertrel™ extraction, polyethylene glyc

SUBMITTER: Philo SE 

PROVIDER: S-EPMC7832770 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature