Development of a loop-mediated isothermal amplification (LAMP) method for specific detection of Mycobacterium bovis.

ABSTRACT: Bovine tuberculosis (TB) caused by Mycobacterium bovis is a significant health threat to cattle and a zoonotic threat for humans in many developing countries. Rapid and accurate detection of M. bovis is fundamental for controlling the disease in animals and humans, and for the proper treatment of patients as one of the first-line anti-TB drug, pyrazinamide, is ineffective against M. bovis. Currently, there are no rapid, simplified and low-cost diagnostic methods that can be easily integrated for use in many developing countries. Here, we report the development of a loop-mediated isothermal amplification (LAMP) assay for specific identification of M. bovis by targeting the region of difference 4 (RD4), a 12.7 kb genomic region that is deleted solely in M. bovis. The assay's specificity was

SUBMITTER: Kapalamula TF 

PROVIDER: S-EPMC7833227 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature