Comparison of commercial realtime reverse transcription PCR assays for the detection of SARS-CoV-2.

ABSTRACT: The emergence of a new coronavirus in Wuhan China has triggered a global need for accurate diagnostic assays. Initially, mostly laboratory developed molecular tests were available but shortly thereafter different commercial assays started to appear and are still increasing in number. Although independent performance evaluations are ongoing, available data is still scarce. Here we provide a direct comparison of key performance characteristics of 13 commercial RT-PCR assays. Thirteen RT-PCR assays were selected based on the criteria that they can be used following generic RNA extraction protocols, on common PCR platforms and availability. Using a 10-fold and 2-fold dilution series of a quantified SARS-CoV-2 cell-cultured virus stock, performance was assessed compared to our in house validate

SUBMITTER: Igloi Z 

PROVIDER: S-EPMC7833356 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature