Dataset Information
Fast SARS-CoV-2 detection by RT-qPCR in preheated nasopharyngeal swab samples.
Ontology highlight
ABSTRACT: Objectives
The gold-standard COVID-19 diagnosis relies on detecting SARS-CoV-2 using RNA purification and one-step retrotranscription and quantitative PCR (RT-qPCR). Based on the urgent need for high-throughput screening, we tested the performance of three alternative, simple and affordable protocols to rapidly detect SARS-CoV-2, bypassing the long and tedious RNA extraction step and reducing the time to viral detection.Methods
We evaluated three methods based on direct nasopharyngeal swab viral transmission medium (VTM) heating before the RT-qPCR: a) direct without additives; b) in a formamide-EDTA (FAE) buffer, c) in a RNAsnapTM buffer.Results
Although with a delay in cycle threshold compared to the gold-standard, we found consistent results in nasopharyngeal swab samples that were subject to a direct 70°C incubation for 10 min.Conclusions
Our findings provide valuable options to overcome any supply chain issue and help to increase the throughput of diagnostic tests, thereby complementing standard diagnosis.
SUBMITTER: Alcoba-Florez J
PROVIDER: S-EPMC7833505 | biostudies-literature | 2020 Aug
REPOSITORIES: biostudies-literature
Publications
Fast SARS-CoV-2 detection by RT-qPCR in preheated nasopharyngeal swab samples.
International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases 20200531
<h4>Objectives</h4>The gold-standard COVID-19 diagnosis relies on detecting SARS-CoV-2 using RNA purification and one-step retrotranscription and quantitative PCR (RT-qPCR). Based on the urgent need for high-throughput screening, we tested the performance of three alternative, simple and affordable protocols to rapidly detect SARS-CoV-2, bypassing the long and tedious RNA extraction step and reducing the time to viral detection.<h4>Methods</h4>We evaluated three methods based on direct nasophary ...[more]