Characterization of CD8⁺ T-cell responses to non-anchor-type HLA class I neoantigens with single amino-acid substitutions.

ABSTRACT: CD8⁺ T cells are capable of recognizing mutation-derived neoantigens displayed by HLA class I molecules, thereby exhibiting the ability to distinguish between cancer and normal cells. However, accumulating evidence has shown that only a small fraction of nonsynonymous somatic mutations give rise to clinically relevant neoantigens. The properties of such neoantigens, which must be presented by HLA and immunogenic to induce a T-cell response, remain elusive. In this study, we explored the HLA class I ligandome of a human cancer cell line with microsatellite instability using a proteogenomic approach. The results demonstrated that neoantigens accounted for only 0.34% of the HLA class I ligandome, and most neoantigens were encoded by genes with abundant expression. Thereafter, T-cel

SUBMITTER: Shinkawa T 

PROVIDER: S-EPMC7833734 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature