In silico prediction and experimental validation of siRNAs targeting ORF1ab of MERS-CoV in Vero cell line.

ABSTRACT: The Middle East Respiratory Syndrome Coronavirus is well known to cause respiratory syndrome and this virus was identified and isolated for the first time from Jeddah, Saudi Arabia in 2012 from infected patient. In this report, we have conducted the in-silico prediction, designing and evaluation of siRNAs targeting Middle East Respiratory Syndrome Coronavirus orf1ab gene to inhibit the virus replication. By using bioinformatics software, total twenty-one functional, off-target reduced siRNA were selected from four hundred and sixty-two siRNAs based on their greater potency and specificity. We have evaluated only seven siRNAs to analyze their performance and efficacy as antivirals by reverse transfection approach in Vero cells. There was no cytotoxicity of siRNAs at various concentra

SUBMITTER: Sohrab SS 

PROVIDER: S-EPMC7833792 | biostudies-literature | 2021 Feb

REPOSITORIES: biostudies-literature