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MiR?93?5p regulates the occurrence and development of esophageal carcinoma epithelial cells by targeting TGF?R2.


ABSTRACT: Emerging studies have indicated that the dysregulation of microRNAs (miRNAs or miRs) plays a vital role in the development and metastasis of tumors. However, the role of miR?93?5p in esophageal carcinoma (EC) has not been extensively reported. The present study thus focused on the role of miR?93?5p and its downstream target in the occurrence and development of EC. Firstly, miRNA expression profiles associated with EC were accessed from the TCGA_ESCA dataset and analyzed. Subsequently, the expression patterns of miR?93?5p and TGF?R2 were characterized in the human esophageal cell line, Het?1A, and the human EC cell lines, TE?1, Eca?109 and EC9706, by RT?qPCR and western blot analysis. WST?1 assay, flow cytometry, Transwell assay, wound healing assay and bioinformatics analysis were used to explore their functions in EC cells. Finally, a dual?luciferase reporter assay was employed to determine the targeted association between miR?93?5p and TGF?R2. The results revealed that the expression of miR?93?5p was markedly higher in EC cell lines compared with that in the normal cell line. The overexpression of miR?93?5p facilitated cell proliferation, migration and invasion, and inhibited cell apoptosis. Additionally, TGF?R2 was identified as a functional target of miR?93?5p in EC cells, as judged by a series of in vitro experiments. Furthermore, it was found that the simultaneous overexpression of miR?93?5p and TGF?R2 almost had no effect on the biological behaviors of EC cells. On the whole, the present study demonstrates that miR?93?5p promotes the proliferation, migration and invasion, and inhibits the apoptosis of EC cells by targeting TGF?R2.

SUBMITTER: Cai Y 

PROVIDER: S-EPMC7834964 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature

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miR‑93‑5p regulates the occurrence and development of esophageal carcinoma epithelial cells by targeting TGFβR2.

Cai Yibin Y   Ruan Weizhong W   Ding Jianming J   Wei Ning N   Wang Jianchao J   Zhang Hong H   Ma Ning N   Weng Guibin G   Su Wei Kun WK   Lin Yijin Y   Zhu Kunshou K  

International journal of molecular medicine 20210115 3


Emerging studies have indicated that the dysregulation of microRNAs (miRNAs or miRs) plays a vital role in the development and metastasis of tumors. However, the role of miR‑93‑5p in esophageal carcinoma (EC) has not been extensively reported. The present study thus focused on the role of miR‑93‑5p and its downstream target in the occurrence and development of EC. Firstly, miRNA expression profiles associated with EC were accessed from the TCGA_ESCA dataset and analyzed. Subsequently, the expres  ...[more]

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