Mycolic acid-containing bacteria trigger distinct types of membrane vesicles through different routes.

ABSTRACT: Bacterial membrane vesicles (MVs) are attracting considerable attention in diverse fields of life science and biotechnology due to their potential for various applications. Although there has been progress in determining the mechanisms of MV formation in Gram-negative and Gram-positive bacteria, the mechanisms in mycolic acid-containing bacteria remain an unsolved question due to its complex cell envelope structure. Here, by adapting super-resolution live-cell imaging and biochemical analysis, we show that Corynebacterium glutamicum form distinct types of MVs via different routes in response to environmental conditions. DNA-damaging stress induced MV formation through prophage-triggered cell lysis, whereas envelope stress induced MV formation through mycomembrane blebbing. The MV fo

SUBMITTER: Nagakubo T 

PROVIDER: S-EPMC7835258 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature