Discovery of Functional Alternatively Spliced PKM Transcripts in Human Cancers.

ABSTRACT: Pyruvate kinase muscle type (PKM) is a key enzyme in glycolysis and plays an important oncological role in cancer. However, the association of PKM expression and the survival outcome of patients with different cancers is controversial. We employed systems biology methods to reveal prognostic value and potential biological functions of PKM transcripts in different human cancers. Protein products of transcripts were shown and detected by western blot and mass spectrometry analysis. We focused on different transcripts of PKM and investigated the associations between their mRNA expression and the clinical survival of the patients in 25 different cancers. We find that the transcripts encoding PKM2 and three previously unstudied transcripts, namely ENST00000389093, EN

SUBMITTER: Li X 

PROVIDER: S-EPMC7835739 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature