Isolation and Characterization of Equine Uterine Extracellular Vesicles: A Comparative Methodological Study.

ABSTRACT: Extracellular vesicles (EVs) have been identified in the uterine fluid in different species and have been pointed as key players in the embryo-maternal dialogue, maternal recognition of pregnancy and establishment of pregnancy. However, little is known about the uterine EVs in the mare. Therefore, the present study aimed at characterizing EVs from uterine lavage of cyclic mares by comparing five EVs isolation methods and the combination of them: (1) ultracentrifugation (UC); (2) concentration of lavage volume by Centricon ultrafiltration (CE); (3) the use of CE with different washing steps (phosphate-buffered saline with or without trehalose); (4) size-exclusion chromatography with iZON-qEV columns, and (5) a combination of the methods with best results based on EVs yield, purity, and prot

SUBMITTER: Alminana C 

PROVIDER: S-EPMC7835857 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature