Synergistic optimisation of expression, folding, and secretion improves E. coli AppA phytase production in Pichia pastoris.

ABSTRACT:

Background

Pichia pastoris (Komagataella phaffii) is an important platform for heterologous protein production due to its growth to high cell density and outstanding secretory capabilities. Recent developments in synthetic biology have extended the toolbox for genetic engineering of P. pastoris to improve production strains. Yet, overloading the folding and secretion capacity of the cell by over-expression of recombinant proteins is still an issue and rational design of strains is critical to achieve cost-effective industrial manufacture. Several enzymes are commercially produced in P. pastoris, with phytases being one of the biggest on the global market. Phytases are ubiquitously used as a dietary supplement for swine and poultry to increase digestibility of phytic acid, the main

SUBMITTER: Navone L 

PROVIDER: S-EPMC7836175 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature