Losartan reduces cigarette smoke-induced airway inflammation and mucus hypersecretion.

ABSTRACT: The aim was to determine whether losartan reduces cigarette smoke (CS)-induced airway inflammation and mucus hypersecretion in an in vitro model and a small clinical trial. Primary human bronchial epithelial cells (HBECs) were differentiated at the air-liquid interface (ALI) and exposed to CS. Expression of transforming growth factor (TGF)-β1 and the mucin MUC5AC, and expression or activity of matrix metalloproteinase (MMP)-9 were measured after CS exposure. Parameters of mucociliary clearance were evaluated by measuring airway surface liquid volumes, mucus concentrations, and conductance of cystic fibrosis transmembrane conductance regulator (CFTR) and large conductance, Ca²⁺-activated and voltage-dependent potassium (BK) channels. Nasal cells were collected from study p

SUBMITTER: Kim MD 

PROVIDER: S-EPMC7836504 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature