Cell membrane rupture: a novel test reveals significant variations among different brands of tissue culture flasks.

ABSTRACT:

Objectives

Loss of cytoplasmic molecules including protein controls, due to cell membrane rupture can cause errors and irreproducibility in research data. Previous results have shown that during the washing of a monolayer of cells with a balanced salt solution, the fluid force causes cell membrane rupture on some areas of the flasks/dishes. This fact shows the non-uniformity of the polystyrene surface in terms of cell culture. There is at present no simple test to monitor that surface. This paper presents a novel biologically based assay to determine the degree of heterogeneity of flasks supplied by various manufacturers.

Results

This paper shows that significant variation exists in polystyrene surface heterogeneity among several brands of tissue culture flasks, varying from

SUBMITTER: Tchao R 

PROVIDER: S-EPMC7836507 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature