Knockdown of NIR Suppresses Breast Cancer Cell Proliferation via Promoting FOXO3.

ABSTRACT:

Background

Novel inhibitor of histone acetyltransferase repressor (NIR), a corepressor with a novel inhibitor of histone acetyltransferase (INHAT) activity, has been reported to be a negative modulator of p53 and a regulator of the cell cycle in cancer cells. However, the role of NIR in the progression of breast cancer remains elusive.

Materials and methods

Oncomine database was used to analyze the mRNA levels and prognosis value of NIR in breast cancer. We performed loss-of-function and gain-of-function studies using lentivirus expressing shRNA targeting NIR, enhancer of zeste homolog 2 (EZH2) and forkhead box O3 (FOXO3) or lentivirus expressing NIR or FOXO3, respectively. Cell proliferation and colony formation assays were performed. Co-immunoprecipitation (Co-IP) and immu

SUBMITTER: Chen B 

PROVIDER: S-EPMC7837597 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature