Analyzing the G3BP-like gene family of Arabidopsis thaliana in early turnip mosaic virus infection.

ABSTRACT: The Arabidopsis thaliana genome encodes several genes that are known or predicted to participate in the formation of stress granules (SG). One family of genes encodes for Ras GTPase-activating protein-binding protein (G3BP)-like proteins. Seven genes were identified, of which one of the members was already shown to interact with plant virus proteins in a previous study. A phylogenetic and tissue-specific expression analysis, including laser-dissected phloem, by qRT-PCRs was performed and the sub-cellular localization of individual AtG3BP::EYFP fluorescent fusion proteins expressed in Nicotiana benthamiana epidermal cells was observed. Individual AtG3BP-protein interactions in planta were studied using the bimolecular fluorescence complementation approach in combination with confocal imagin

SUBMITTER: Reuper H 

PROVIDER: S-EPMC7838295 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature