A THP-1 Cell Line-Based Exploration of Immune Responses Toward Heat-Treated BLG.

ABSTRACT: Allergen recognition and processing by antigen presenting cells is essential for the sensitization step of food allergy. Macrophages and dendritic cells are both phagocytic antigen presenting cells and play important roles in innate immune responses and signaling between the innate and adaptive immune system. To obtain a model system with a homogeneous genetic background, we derived macrophages and dendritic cells from THP-1 monocytes. The difference between macrophages and dendritic cells was clearly shown by differences in their transcription response (microarray) and protein expression levels. Their resemblance to primary cells was analyzed by comparison to properties as described in literature. The uptake of β-lactoglobulin after wet-heating (60°C in solution) by THP-1 derived macropha

SUBMITTER: Deng Y 

PROVIDER: S-EPMC7838438 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature