Intestinal TMEM16A control luminal chloride secretion in a NHERF1 dependent manner.

ABSTRACT: TMEM16A (Transmembrane protein 16A or Anoctamin1) is a calcium-activated chloride channel. (CaCC),that exerts critical roles in epithelial secretion. However, its localization, function, and regulation in intestinal chloride (Cl^-) secretion remain obscure. Here, we show that TMEM16A protein abundance correlates with Cl^- secretion in different regions of native intestine activated by the Ca²⁺-elevating muscarinic agonist carbachol (CCH). Basal, as well as both cAMP- and CCH-stimulated Isc, was largely reduced in Ano1 ± mouse intestine. We found CCH was not able to increase Isc in the presence of apical to serosal Cl^- gradient, strongly supporting TMEM16A as primarily a luminal Cl^- channel. Immunostaining demonstrated apical local

SUBMITTER: Saha T 

PROVIDER: S-EPMC7838733 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature