Polymorphism Analysis of <i>pfmdr1</i> and <i>pfcrt</i> from <i>Plasmodium falciparum</i> Isolates in Northwestern Nigeria Revealed the Major Markers Associated with Antimalarial Resistance.

ABSTRACT: Suspicion of failure in the effectiveness of artemisinin-based combination therapies (currently the first-line treatment of malaria, worldwide) is leading to the unofficial use of alternative antimalarials, including chloroquine and sulfadoxine/pyrimethamine, across northern Nigeria. To facilitate evidence-based resistance management, antimalarial resistance mutations were investigated in Plasmodium falciparum multidrug resistance-1 (pfmdr1) and chloroquine resistance transporter (pfcrt), in isolates from Kano, northwestern Nigeria. Out of the 88 samples genotyped for pfmdr1N86Y mutation using PCR/restriction fragment length polymorphism, one sample contained the 86Y mutation (86Y_frequency = 1.14%). The analysis of 610 bp fragments of pfmdr1

SUBMITTER: Adam R 

PROVIDER: S-EPMC7838797 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature