Purification and Characterization of Recombinant Expressed Apple Allergen Mal d 1.

ABSTRACT: Mal d 1 is the primary apple allergen in northern Europe. To explain the differences in the allergenicity of apple varieties, it is essential to study its properties and interaction with other phytochemicals, which might modulate the allergenic potential. Therefore, an optimized production route followed by an unsophisticated purification step for Mal d 1 and respective mutants is desired to produce sufficient amounts. We describe a procedure for the transformation of the plasmid in competent E. coli cells, protein expression and rapid one-step purification. r-Mal d 1 with and without a polyhistidine-tag are purified by immobilized metal ion affinity chromatography (IMAC) and fast-protein liquid chromatography (FPLC) using a high-resolution anion-exchange column, respectively. Purity is es

SUBMITTER: Kaeswurm JAH 

PROVIDER: S-EPMC7838873 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature