Quantitative planar array screen of 1000 proteins uncovers novel urinary protein biomarkers of lupus nephritis.

ABSTRACT:

Objective

The goal of these studies is to discover novel urinary biomarkers of lupus nephritis (LN).

Methods

Urine from systemic lupus erythematosus (SLE) patients was interrogated for 1000 proteins using a novel, quantitative planar protein microarray. Hits were validated in an independent SLE cohort with inactive, active non-renal (ANR) and active renal (AR) patients, in a cohort with concurrent renal biopsies, and in a longitudinal cohort. Single-cell renal RNA sequencing data from LN kidneys were examined to deduce the cellular origin of each biomarker.

Results

Screening of 1000 proteins revealed 64 proteins to be significantly elevated in SLE urine, of which 17 were ELISA validated in independent cohorts. Urine Angptl4 (area under the curve (AUC)=0.96), L-selecti

SUBMITTER: Vanarsa K 

PROVIDER: S-EPMC7839323 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature