Rapid removal of phagosomal ferroportin in macrophages contributes to nutritional immunity.

ABSTRACT: Nutrient sequestration is an essential facet of host innate immunity. Macrophages play a critical role in controlling iron availability through expression of the iron transport protein ferroportin (FPN), which extrudes iron from the cytoplasm to the extracellular milieu. During phagocytosis, the limiting phagosomal membrane, which derives from the plasmalemma, can be decorated with FPN and, if functional, will move iron from the cytosol into the phagosome lumen. This serves to feed iron to phagocytosed microbes and would be counterproductive to the many other known host mechanisms working to starve microbes of this essential metal. To understand how FPN is regulated during phagocytosis, we expressed FPN as a green fluorescent protein-fusion protein in macrophages and monitored its localiza

SUBMITTER: Flannagan RS 

PROVIDER: S-EPMC7839378 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature