Galectin-Glycan Interactions: Guidelines for Monitoring by ⁷⁷ Se NMR Spectroscopy, and Solvent (H₂ O/D₂ O) Impact on Binding.

ABSTRACT: Functional pairing between cellular glycoconjugates and tissue lectins like galectins has wide (patho)physiological significance. Their study is facilitated by nonhydrolysable derivatives of the natural O-glycans, such as S- and Se-glycosides. The latter enable extensive analyses by specific ⁷⁷ Se NMR spectroscopy, but still remain underexplored. By using the example of selenodigalactoside (SeDG) and the human galectin-1 and -3, we have evaluated diverse ⁷⁷ Se NMR detection methods and propose selective ¹ H,⁷⁷ Se heteronuclear Hartmann-Hahn transfer for efficient use in competitive NMR screening against a selenoglycoside spy ligand. By fluorescence anisotropy, circular dichroism, and isothermal titration calorimetry (ITC), we show that the affini

SUBMITTER: Diercks T 

PROVIDER: S-EPMC7839768 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature