Investigation of factors associated with ABCB5-positive limbal stem cell isolation yields from human donors.

ABSTRACT:

Purpose

To identify factors associated with isolation yields of ATP-binding cassette (ABC) superfamily member B5 (ABCB5)-positive limbal stem cells (LSCs) from human cadaveric donor eyes.

Methods

Whole eye globes were obtained from the Saving Sight eye bank, Kansas City, MO and the CorneaGen eye bank, Seattle, WA. ABCB5-positive LSCs were sorted by flow cytometry upon anti-ABCB5 monoclonal antibody staining within one week after donor death. The yields of live limbal epithelial cells in their entirety and of isolated pure ABCB5-positive LSC subsets were correlated with variables contained in the eye donors' medical information.

Results

The mean isolation yield of live limbal epithelial cells and ABCB5-positive LSCs per donor eye was (340,000 ± 160,000 and 2,608 ± 1,84

SUBMITTER: Sasamoto Y 

PROVIDER: S-EPMC7840156 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature