High-throughput toxicogenomic screening of chemicals in the environment using metabolically competent hepatic cell cultures.

ABSTRACT: The ToxCast in vitro screening program has provided concentration-response bioactivity data across more than a thousand assay endpoints for thousands of chemicals found in our environment and commerce. However, most ToxCast screening assays have evaluated individual biological targets in cancer cell lines lacking integrated physiological functionality (such as receptor signaling, metabolism). We evaluated differentiated HepaRG^TM cells, a human liver-derived cell model understood to effectively model physiologically relevant hepatic signaling. Expression of 93 gene transcripts was measured by quantitative polymerase chain reaction using Fluidigm 96.96 dynamic arrays in response to 1060 chemicals tested in eight-point concentration-response. A Bayesian framework quantitatively mod

SUBMITTER: Franzosa JA 

PROVIDER: S-EPMC7840683 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature