The carboxy terminal coiled-coil modulates Orai1 internalization during meiosis.

ABSTRACT: Regulation of Ca²⁺ signaling is critical for the progression of cell division, especially during meiosis to prepare the egg for fertilization. The primary Ca²⁺ influx pathway in oocytes is Store-Operated Ca²⁺ Entry (SOCE). SOCE is tightly regulated during meiosis, including internalization of the SOCE channel, Orai1. Orai1 is a four-pass membrane protein with cytosolic N- and C-termini. Orai1 internalization requires a caveolin binding motif (CBM) in the N-terminus as well as the C-terminal cytosolic domain. However, the molecular determinant for Orai1 endocytosis in the C-terminus are not known. Here we show that the Orai1 C-terminus modulates Orai1 endocytosis during meiosis through a structural motif that is based on the strength of the C-terminal inters

SUBMITTER: Hodeify R 

PROVIDER: S-EPMC7840751 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature