Mapping the immune environment in clear cell renal carcinoma by single-cell genomics.

ABSTRACT: Clear cell renal cell carcinoma (ccRCC) is one of the most immunologically distinct tumor types due to high response rate to immunotherapies, despite low tumor mutational burden. To characterize the tumor immune microenvironment of ccRCC, we applied single-cell-RNA sequencing (SCRS) along with T-cell-receptor (TCR) sequencing to map the transcriptomic heterogeneity of 25,688 individual CD45⁺ lymphoid and myeloid cells in matched tumor and blood from three patients with ccRCC. We also included 11,367 immune cells from four other individuals derived from the kidney and peripheral blood to facilitate the identification and assessment of ccRCC-specific differences. There is an overall increase in CD8⁺ T-cell and macrophage populations in tumor-infiltrated immune cells com

SUBMITTER: Borcherding N 

PROVIDER: S-EPMC7840906 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature