Growth, lifetime, directional movement and myosin-dependent motility of mutant keratin granules in cultured cells.

ABSTRACT: Intermediate filament polypeptides (IFPs) are prominent components of cytoplasmic aggregates, which are pathognomonic for multiple diseases. Recent observations in cultured cells suggest that they are dynamic and subject to regulated turnover. The emerging concept is that multiple factors contribute to motility and turnover of IFP-containing aggregates. To understand their relative contribution, quantitative tools are needed. The current study addresses this need using epithelial cells producing mutant keratin IFPs that have been identified as the cause of the hereditary blister-forming skin disease epidermolysis bullosa simplex. Digital image analysis of individual granules allowed mapping of their complete life cycle, with information on multiple characteristics at any given time-point.

SUBMITTER: Lehmann SM 

PROVIDER: S-EPMC7840912 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature