Requirement of splicing factor hnRNP A2B1 for tumorigenesis of melanoma stem cells.

ABSTRACT:

Background

Cancer stem cells play essential roles in tumorigenesis, thus forming an important target for tumor therapy. The hnRNP family proteins are important splicing factors that have been found to be associated with tumor progression. However, the influence of hnRNPs on cancer stem cells has not been extensively explored.

Methods

Quantitative real-time PCR and Western blot were used to examine gene expressions. RNA immunoprecipitation assays were conducted to identify the RNAs interacted with hnRNP A2B1. The in vivo assays were performed in nude mice.

Results

In this study, the results showed that out of 19 evaluated hnRNPs, hnRNP A2B1 was significantly upregulated in melanoma stem cells compared with non-stem cells, suggesting an important role of hnRNP A2B1 in c

SUBMITTER: Chu M 

PROVIDER: S-EPMC7842053 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature