Higher genome variability within metabolism genes associates with recurrent Clostridium difficile infection.

ABSTRACT:

Background

Clostridium difficile (C. difficile) is a major source of healthcare-associated infection with a high risk of recurrence, attributable to many factors such as usage of antibiotics, older age and immunocompromised status of the patients. C. difficile has also a highly diverse genome, which may contribute to its high virulence. Herein we examined whether the genome conservation, measured as non-synonymous to synonymous mutations ratio (dN/dS) in core genes, presence of single genes, plasmids and prophages increased the risk of reinfection in a subset of 134 C. difficile isolates from our previous study in a singly hemato-oncology ward.

Methods

C. difficile isolates were subjected to whole-genome sequencing (WGS) on Ion Torrent PGM sequencer. Genomes were assembled w

SUBMITTER: Kulecka M 

PROVIDER: S-EPMC7842062 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature