Tryp-N: A Thermostable Protease for the Production of N-terminal Argininyl and Lysinyl Peptides.

ABSTRACT: Bottom-up proteomics is a mainstay in protein identification and analysis. These studies typically employ proteolytic treatment of biological samples to generate suitably sized peptides for tandem mass spectrometric (MS) analysis. In MS, fragmentation of peptides is largely driven by charge localization. Consequently, peptides with basic centers exclusively on their N-termini produce mainly b-ions. Thus, it was long ago realized that proteases that yield such peptides would be valuable proteomic tools for achieving simplified peptide fragmentation patterns and peptide assignment. Work by several groups has identified such proteases, however, structural analysis of these suggested that enzymatic optimization was possible. We therefore endeavored to find enzymes that could provide enhanced a

SUBMITTER: Wilson JP 

PROVIDER: S-EPMC7842235 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature