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Quantitative Nucleocytoplasmic Transport Assays in Cellular Models of Neurodegeneration.

ABSTRACT: Nucleocytoplasmic transport deficits are suggested to play a role in neurodegenerative disorders, including amyotrophic lateral sclerosis (ALS). Given the importance and complexity of this process, understanding when these aberrations occur and which pathways are involved is of great importance. Here, we make use of CRISPR-Cas9 technology to design cell lines stably expressing fluorophore proteins shuttling between the nucleus and cytoplasm by karyopherins of choice. To validate this protocol, we measured an ALS-associated nucleocytoplasmic transport pathway in the presence of the disease-associated peptide poly-PR. This technique allows measuring a particular active nucleocytoplasmic transport pathway in intact cells in a neurodegenerative disease-associated context. Moreover, these experiments can be performed without the need for expensive equipment and have the potential to be upscaled for high-throughput screening purposes.

SUBMITTER: Vanneste J

PROVIDER: S-EPMC7842572 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature

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Quantitative Nucleocytoplasmic Transport Assays in Cellular Models of Neurodegeneration.

Vanneste Joni J Vercruysse Thomas T Van Damme Philip P Van Den Bosch Ludo L Daelemans Dirk D

Bio-protocol 20200620 12

Nucleocytoplasmic transport deficits are suggested to play a role in neurodegenerative disorders, including amyotrophic lateral sclerosis (ALS). Given the importance and complexity of this process, understanding when these aberrations occur and which pathways are involved is of great importance. Here, we make use of CRISPR-Cas9 technology to design cell lines stably expressing fluorophore proteins shuttling between the nucleus and cytoplasm by karyopherins of choice. To validate this protocol, w ...[more]

PMID: 33659329

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