Quantitative Nucleocytoplasmic Transport Assays in Cellular Models of Neurodegeneration.

ABSTRACT: Nucleocytoplasmic transport deficits are suggested to play a role in neurodegenerative disorders, including amyotrophic lateral sclerosis (ALS). Given the importance and complexity of this process, understanding when these aberrations occur and which pathways are involved is of great importance. Here, we make use of CRISPR-Cas9 technology to design cell lines stably expressing fluorophore proteins shuttling between the nucleus and cytoplasm by karyopherins of choice. To validate this protocol, we measured an ALS-associated nucleocytoplasmic transport pathway in the presence of the disease-associated peptide poly-PR. This technique allows measuring a particular active nucleocytoplasmic transport pathway in intact cells in a neurodegenerative disease-associated context. Moreover, these exper

SUBMITTER: Vanneste J 

PROVIDER: S-EPMC7842572 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature