Whole-genome Identification of Transcriptional Start Sites by Differential RNA-seq in Bacteria.

ABSTRACT: Gene transcription in bacteria often starts some nucleotides upstream of the start codon. Identifying the specific Transcriptional Start Site (TSS) is essential for genetic manipulation, as in many cases upstream of the start codon there are sequence elements that are involved in gene expression regulation. Taken into account the classical gene structure, we are able to identify two kinds of transcriptional start site: primary and secondary. A primary transcriptional start site is located some nucleotides upstream of the translational start site, while a secondary transcriptional start site is located within the gene encoding sequence. Here, we present a step by step protocol for genome-wide transcriptional start sites determination by differential RNA-sequencing (dRNA-seq) using the enter

SUBMITTER: Cervantes-Rivera R 

PROVIDER: S-EPMC7842792 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature