Low-cost and High-throughput RNA-seq Library Preparation for Illumina Sequencing from Plant Tissue.

ABSTRACT: Transcriptome analysis can provide clues to biological processes affected in different genetic backgrounds or/and under various conditions. The price of RNA sequencing (RNA-seq) has decreased enough so that medium- to large-scale transcriptome analyses in a range of conditions are feasible. However, the price and variety of options for library preparation of RNA-seq can still be daunting to those who would like to use RNA-seq for their first time or for a single experiment. Among the criteria for selecting a library preparation protocol are the method of RNA isolation, nucleotide fragmentation to obtain desired size range, and library indexing to pool sequencing samples for multiplexing. Here, we present a high-quality and a high-throughput option for preparing libraries from polyadenylate

SUBMITTER: Bjornson M 

PROVIDER: S-EPMC7842799 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature