Assessing Self-interaction of Mammalian Nuclear Proteins by Co-immunoprecipitation.

ABSTRACT: Protein-protein interactions constitute the molecular foundations of virtually all biological processes. Co-immunoprecipitation (CoIP) experiments are probably the most widely used method to probe both heterotypic and homotypic protein-protein interactions. Recent advances in super-resolution microscopy have revealed that several nuclear proteins such as transcription factors are spatially distributed into local high-concentration clusters in mammalian cells, suggesting that many nuclear proteins self-interact. These observations have further underscored the need for orthogonal biochemical approaches for testing if self-association occurs, and if so, what the mechanisms are. Here, we describe a CoIP protocol specifically optimized to test self-association of endogenously tagged nuclear pro

SUBMITTER: Cattoglio C 

PROVIDER: S-EPMC7842838 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature