Comparing lifeact and phalloidin for super-resolution imaging of actin in fixed cells.

ABSTRACT: Visualizing actin filaments in fixed cells is of great interest for a variety of topics in cell biology such as cell division, cell movement, and cell signaling. We investigated the possibility of replacing phalloidin, the standard reagent for super-resolution imaging of F-actin in fixed cells, with the actin binding peptide 'lifeact'. We compared the labels for use in single molecule based super-resolution microscopy, where AlexaFluor 647 labeled phalloidin was used in a dSTORM modality and Atto 655 labeled lifeact was used in a single molecule imaging, reversible binding modality. We found that imaging with lifeact had a comparable resolution in reconstructed images and provided several advantages over phalloidin including lower costs, the ability to image multiple regions of interest on

SUBMITTER: Mazloom-Farsibaf H 

PROVIDER: S-EPMC7842966 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature