Comparison of four commercial RT-PCR diagnostic kits for COVID-19 in China.

ABSTRACT: We compared the sensitivity and specificity of four commercial coronavirus disease (COVID-19) diagnostic kits using real-time reverse transcription-polymerase chain reaction (RT-PCR). Kits I-IV approved by the State Drug Administration of China were selected, and the detection targets were ORF1ab gene and N gene. Specificity was evaluated by detecting other respiratory viruses. The sensitivity and batch effect of each kit were evaluated by testing 10-fold dilutions of RNA. Clinical application was verified by testing nasopharyngeal swab and sputum specimens from COVID-19 patients. Among the 78 cases infected by other respiratory viruses, no amplification curve was observed using these four COVID-19 RT-PCR kits. The minimum detection limits of kits I-IV were 10^-6 , 10^-5

SUBMITTER: Shen L 

PROVIDER: S-EPMC7843276 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature