Gene set enrichment analysis of α-amylase and α-glucosidase inhibitors of Cassia glauca.

ABSTRACT:

Background

The present study aimed to evaluate in vitro α-amylase and α-glucosidase inhibitory activity of various extracts of Cassia glauca, predict the binding affinity of multiple phytoconstituents with both enzymes via in silico molecular docking and identify the probably modulated pathways by the lead hit.

Methods

Different extracts of Cassia glauca i.e. acetone, ethanol, and aqueous extracts were evaluated for α-amylase and α-glucosidase inhibitory activity using in vitro method in which starch and 4-Nitrophenyl β-D-glucopyranoside were used as substrate respectively. Similarly, the docking study was performed using autodock4 to predict the binding affinity of phytoconstituents with α-amylase and α-glucosidase. After docking, ten different p

SUBMITTER: Ternikar SG 

PROVIDER: S-EPMC7843725 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature