Mechanistic dissection of diabetic retinopathy using the protein-metabolite interactome.

ABSTRACT:

Purpose

The current study aims to determine the molecular mechanisms of diabetic retinopathy (DR) using the protein-protein interactome and metabolome map. We examined the protein network of novel biomarkers of DR for direct (physical) and indirect (functional) interactions using clinical target proteins in different models.

Methods

We used proteomic tools including 2-dimensional gel electrophoresis, mass spectrometry analysis, and database search for biomarker identification using in vivo murine and human model of diabetic retinopathy and in vitro model of oxidative stress. For the protein interactome and metabolome mapping, various bioinformatic tools that include STRING and OmicsNet were used.

Results

We uncovered new diabetic biomarkers including prohibitin (PHB),

SUBMITTER: Patrick AT 

PROVIDER: S-EPMC7843730 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature