A translation enhancer element from black beetle virus engages yeast eIF4G1 to drive cap-independent translation initiation.

ABSTRACT: Cap-independent translation initiation plays crucial roles in fine-tuning gene expression under global translation shutdown conditions. Translation of uncapped or de-capped transcripts can be stimulated by Cap-independent translation enhancer (CITE) elements, but the mechanisms of CITE-mediated translation initiation remain understudied. Here, we characterized a short 5'-UTR RNA sequence from black beetle virus, BBV-seq. Mutational analysis indicates that the entire BBV-seq is required for efficient translation initiation, but this sequence does not operate as an IRES-type module. In yeast cell-free translation extracts, BBV-seq promoted efficient initiation on cap-free mRNA using a scanning mechanism. Moreover, BBV-seq can increase translation efficiency resulting from conventional cap-de

SUBMITTER: Trainor BM 

PROVIDER: S-EPMC7844027 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature