Translational control of enzyme scavenger expression with toxin-induced micro RNA switches.

ABSTRACT: Biological computation requires in vivo control of molecular behavior to progress development of autonomous devices. miRNA switches represent excellent, easily engineerable synthetic biology tools to achieve user-defined gene regulation. Here we present the construction of a synthetic network to implement detoxification functionality. We employed a modular design strategy by engineering toxin-induced control of an enzyme scavenger. Our miRNA switch results show moderate synthetic expression control over a biologically active detoxification enzyme molecule, using an established design protocol. However, following a new design approach, we demonstrated an evolutionarily designed miRNA switch to more effectively activate enzyme activity than synthetically designed versions, allowing markedly

SUBMITTER: Pollak NM 

PROVIDER: S-EPMC7844233 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature