Molecular organization of cytokinesis node predicts the constriction rate of the contractile ring.

ABSTRACT: The molecular organization of cytokinesis proteins governs contractile ring function. We used single molecule localization microscopy in live cells to elucidate the molecular organization of cytokinesis proteins and relate it to the constriction rate of the contractile ring. Wild-type fission yeast cells assemble contractile rings by the coalescence of cortical proteins complexes called nodes whereas cells without Anillin/Mid1p (Δmid1) lack visible nodes yet assemble contractile rings competent for constriction from the looping of strands. We leveraged the Δmid1 contractile ring assembly mechanism to determine how two distinct molecular organizations, nodes versus strands, can yield functional contractile rings. Contrary to previous interpretations, nodes assemble in Δmid1 cells. Our resul

SUBMITTER: Bellingham-Johnstun K 

PROVIDER: S-EPMC7844425 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature