The effect of specific binding proteins on immunoassay measurements of total and free thyroid hormones and cortisol.

ABSTRACT:

Background

Immunoassay (IA) measurements of thyroid hormones have previously given inaccurate results of triiodothyronine (T3), free triiodothyronine (FT3), and free thyroxine (FT4) when concentrations of TBG are low. We evaluate the hypothesis that abnormal concentrations of specific binding proteins (BPs) affect IA measurements and provide results which might misguide the diagnosis and treatment of patients. This study assesses IAs for the measurement of T3, FT3, and cortisol when levels of TBG and CBG are high or low. Comparisons are made between IA and LC-MS/MS.

Methods

Serum or plasma samples with high (>95th percentile, n = 25) or low (<5th percentile, n = 27) concentrations of BP were collected. The concentrations of T3, FT3, and cortisol were measured b

SUBMITTER: Gant Kanegusuku A 

PROVIDER: S-EPMC7844449 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature