Development of multivalent nanobodies blocking SARS-CoV-2 infection by targeting RBD of spike protein.

ABSTRACT:

Background

The outbreak and pandemic of coronavirus SARS-CoV-2 caused significant threaten to global public health and economic consequences. It is extremely urgent that global people must take actions to develop safe and effective preventions and therapeutics. Nanobodies, which are derived from single‑chain camelid antibodies, had shown antiviral properties in various challenge viruses. In this study, multivalent nanobodies with high affinity blocking SARS-CoV-2 spike interaction with ACE2 protein were developed.

Results

Totally, four specific nanobodies against spike protein and its RBD domain were screened from a naïve VHH library. Among them, Nb91-hFc and Nb3-hFc demonstrated antiviral activity by neutralizing spike pseudotyped viruses in vitro. Subsequently, multivalent

SUBMITTER: Lu Q 

PROVIDER: S-EPMC7844813 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature