Analysis of virulence factors and antibiotic resistance genes in group B streptococcus from clinical samples.

ABSTRACT:

Background

Streptococcus agalacticae (Group B Streptococcus, GBS) is one of the most important causative agents of serious infections among neonates. This study was carried out to identify antibiotic resistance and virulence genes associated with GBS isolated from pregnant women.

Methods

A total of 43 GBS isolates were obtained from 420 vaginal samples collected from HIV positive and negative women who were 13-35 weeks pregnant attending Antenatal Care at Chitungwiza and Harare Central Hospitals in Zimbabwe. Identification tests of GBS isolates was done using standard bacteriological methods and molecular identification testing. Antibiotic susceptibility testing was done using the modified Kirby-Bauer method and E-test strips. The boiling method was used to extract DNA and P

SUBMITTER: Mudzana R 

PROVIDER: S-EPMC7844887 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature