Purification and identification of a surfactin biosurfactant and engine oil degradation by Bacillus velezensis KLP2016.

ABSTRACT: Engine oil used in automobiles is a threat to soil and water due to the recalcitrant properties of its hydrocarbons. It pollutes surrounding environment which affects both flora and fauna. Microbes can degrade hydrocarbons containing engine oil and utilize it as a substrate for their growth. Our results demonstrated that cell-free broth of Bacillus velezensis KLP2016 (Gram + ve, endospore forming; Accession number KY214239) recorded an emulsification index (E₂₄%) from 52.3% to 65.7% against different organic solvents, such as benzene, pentane, cyclohexane, xylene, n-hexane, toluene and engine oil. The surface tension of the cell-free broth of B. velezensis grown in Luria-Bertani broth at 35 °C decreased from 55 to 40 mN m^-1at critical micelle concentration 17.2 µg/mL.

SUBMITTER: Meena KR 

PROVIDER: S-EPMC7844888 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature