The canine activated platelet secretome (CAPS): A translational model of thrombin-evoked platelet activation response.

ABSTRACT:

Background

Domestic dogs represent a translational animal model to study naturally occurring human disease. Proteomics has emerged as a promising tool for characterizing human platelet pathophysiology; thus a detailed characterization of the core canine activated platelet secretome (CAPS) will enhance utilization of the canine model. The objectives of this study were development of a robust, high throughput, label-free approach for proteomic identification and quantification of the canine platelet (i) thrombin releasate proteins, and (ii) the protein subgroup that constitutes CAPS.

Methods

Platelets were isolated from 10 healthy dogs and stimulated with 50 nmol/L of γ-thrombin or saline. Proteins were in-solution trypsin-digested and analyzed by nano-liquid chromatography-ta

SUBMITTER: Cremer SE 

PROVIDER: S-EPMC7845059 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature