Forming megakaryocytes from murine-induced pluripotent stem cells by the inducible overexpression of supporting factors.

ABSTRACT:

Background

Platelets are small anucleate cells that circulate in the blood in a resting state but can be activated by external cues. In case of need, platelets from blood donors can be transfused. As an alternative source, platelets can be produced from induced pluripotent stem cells (iPSCs); however, recovered numbers are low.

Objectives

To optimize megakaryocyte (MK) and platelet output from murine iPSCs, we investigated overexpression of the transcription factors GATA-binding factor 1 (GATA1); nuclear factor, erythroid 2; and pre-B-cell leukemia transcription factor 1 (Pbx1) and a hyperactive variant of the small guanosine triphosphatase RhoA (RhoAhc).

Methods

To avoid off-target effects, we generated iPSCs carrying the reverse tetracycline-responsive transactivato

SUBMITTER: Cullmann K 

PROVIDER: S-EPMC7845061 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature