Simultaneous detection and ribotyping of Clostridioides difficile, and toxin gene detection directly on fecal samples.

ABSTRACT:

Background

Clostridioides difficile is the most common cause of nosocomial diarrhea. Ribotyping of cultured strains by a PCR-based test is used to study potential transmission between patients. We aimed to develop a rapid test that can be applied directly on fecal samples for simultaneous detection and ribotyping of C. difficile, as well as detection of toxin genes.

Methods

We developed a highly specific and sensitive primer set for simultaneous detection and ribotyping of C. difficile directly on total fecal DNA. Toxin genes were detected with primers adapted from Persson et al. (Clin Microbiol Infect 14(11):1057-1064). Our study set comprised 130 fecal samples: 65 samples with positive qPCR for C. difficile toxin A/B genes and 65 C. difficile qPCR negative samples. PCR pro

SUBMITTER: van Rossen TM 

PROVIDER: S-EPMC7845108 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature