A comprehensive enhancer screen identifies TRAM2 as a key and novel mediator of YAP oncogenesis.

ABSTRACT:

Background

Frequent activation of the co-transcriptional factor YAP is observed in a large number of solid tumors. Activated YAP associates with enhancer loci via TEAD4-DNA-binding protein and stimulates cancer aggressiveness. Although thousands of YAP/TEAD4 binding-sites are annotated, their functional importance is unknown. Here, we aim at further identification of enhancer elements that are required for YAP functions.

Results

We first apply genome-wide ChIP profiling of YAP to systematically identify enhancers that are bound by YAP/TEAD4. Next, we implement a genetic approach to uncover functions of YAP/TEAD4-associated enhancers, demonstrate its robustness, and use it to reveal a network of enhancers required for YAP-mediated proliferation. We focus on Enhancer^TRAM2

SUBMITTER: Li L 

PROVIDER: S-EPMC7845134 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature